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Image Search Results
Journal: Bioactive Materials
Article Title: DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy
doi: 10.1016/j.bioactmat.2021.12.001
Figure Lengend Snippet: Characterization of microparticles . (A) For the various particles, SEM images and confocal fluorescence images were obtained for DNA, anti-CD28 antibody (CD28Ab), anti-CD3 antibody (CD3Ab), and hIL2 staining. Scale bar: 0.5 μm. (B) Sizes of microparticles were measured by dynamic light scattering. (C, D) Loading amount of hIL2 (C) and anti-CD3 and anti-CD28 antibodies (D) in each sample was measured by ELISA. ***: significantly different from the other groups at P < 0.001; n.s.: not significantly different (P > 0.05).
Article Snippet: The supernatant was collected and the amount of released hIL2 was measured using a
Techniques: Fluorescence, Staining, Enzyme-linked Immunosorbent Assay
Journal: Bioactive Materials
Article Title: DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy
doi: 10.1016/j.bioactmat.2021.12.001
Figure Lengend Snippet: In vitro T cell activation by DM-AI. (A) Naïve T cells were stained with CFSE dye and proliferation was examined 4 days after treatments with various particles. CD3 + CD8 + T cells and CD3 + CD4 + T cells were gated for CFSE signal analysis. (B) The fold expansion of each T cell population was measured by CFSE assay. (C) Treg cell differentiation was analyzed as the population of CD4 + CD25 + FoxP3 + . (D) The ratio of Treg cells to total CD4 T cells. (E, F) Supernatants of T cells treated with the various formulations were analyzed for IL2 (E) and IFN-γ (F). ***: significantly different at P < 0.001; n.s.: not significantly different (P > 0.05).
Article Snippet: The supernatant was collected and the amount of released hIL2 was measured using a
Techniques: In Vitro, Activation Assay, Staining, CFSE Assay, Cell Differentiation